Experiment Table CD4+ T lymphocyte counts and viral RNA Results Tetramer Staining Results Neutralizing Antibody Assays Results Viral Sequencing Results Peptide-binding Assays Results ELISPOT assays Results Conclusion   Navigation Home Methods Results Conclusion Quiz Links

Peptide Binding Assay Results This test measured the inhibition of binding of iodinated p11C peptides to cells containing a Mamu-A*01 MHC I allele. This demonstrates the relative affinity of the test peptide (mutant p11C) to the Mamu-A*01 MHC I compared to the wild type p11C. The p11B protein is a negative control that should show no binding inhibition. The wild type p11C, at all levels, showed greater inhibition of binding than did the mutant form. It was determined that the mutant form had greater than 100 fold lower affinity for Mamu-A*01 MHC I than did the wild type.  From the graph, how does p11C compare with p11C* (mutant)? What conclusions could you draw from this? Answer The test peptide (competitor) for p11C is having a more difficult time binding than the competitor for mutant p11C. Thus p11C bound more and has higher affinity   NEXT