|
Tetramer
Staining
Synopsis: This assay utilizes antibodies
conjugated with various molecular tags. This technique was
used for determing the number of CD8 cells in the
circulation that detect certain epitopes on the various HIV
antigens. This assay can show the researchers which of these
epitopes appear to be elicited an immune response and if
there are any differences between the responses and which
epitopes appear to play the greatest role in conferring
immunity.
Procedure:
A blood sample is taken from the test subject
The whole blood is satined with the antibodies and
reagents to tag certain molecules.
For this experiment the following reagent were
used:
Fluorescein labeled anti-human CD8-Alpha
ECD labeled anti-human CD8-alpha-beta
APC-labeled anti-rhesus-monkey CD3 monoclonal
antibodies to stain epitope specific CD8 cells
The blood cells are then lysed, washed, and fixed.
These samples were then analyzed by four color flow
cytometry.
|