Are reovirus particles lacking genomic dsRNA capable of inducing apoptosis?

RESULTS

Reovirus mutants lacking double stranded RNA are capable of inducing apoptosis.

Apoptosis induced by dsRNA^- ISVPs increases as the MOI increases.

QUESTIONS

The experiment in which HeLa cells were infected with dsRNA^- ISVPs at different MOIs (figure B) was performed to see if:

A. The dsRNA- mutant could elicit an apoptotic effect. Incorrect.

B. Apoptosis is dependent on the concentration dsRNA- reovirus. Correct.

C. No reason, this was another assay to confirm results. Incorrect.

EXPLANATION This assay was performed to explain why dsRNA- mutants did not induce apoptosis as efficiently as the dsRNA+ virions. These results demonstrated that the apoptotic response is determined by the viral dose.

The results of these experiments proved that steps in viral replication subsequent to penetration of cellular membranes are dispensable for reovirus-induced apoptosis.

TRUE Correct.

FALSE Incorrect.

EXPLANATION These results demonstrated that events following disassembly are not required for apoptosis, which is why apoptosis still occurs even in viruses lacking dsRNA.

CONCLUSION

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