TERMS

Anti-IgM: surface immunoglobin; functions to induce the lytic cycle.

B memory cells: lymphocytes that are produced in the bone marrow are important in antibody response; persist in the body for years and give prompt response if same antigen is encountered again at a later time.

Burkitt's Lymphoma (BL): a B cell lymphoma and EBV is known to transform B cells; a cancerous growth arising in the lymphatic system and spreading to the jaw, eye socket, abdominal organs, and ovaries.

BZLF-1: an EBV lytic protein that modifies cell cycle regulation by inducing cell cycle arrest.

Caspase: a protein that causes a cell to commit suicide (apoptosis).

Ceramide:Ceramide is a complex lipid which can be found in the cytoplasm and the plasma membrane. It is produced by the breakdown of sphingomyelin by sphingomyelinases, a process which is enhanced during TNF and CD 95 ligand induced cell death. It appears that ceramide acts as a second messenger which can mediate apoptosis by the activation of certain kinases.

Cisplatin:a DNA damaging anticancer drug used most successfully for the treatment of testicular tumors. The toxicity of cisplatin likely results from its ability to form covalent DNA adducts that inhibit polymerases.

EBNA-1: one of six EBV nuclear antigens that together with three membrane proteins activate quiescent B cells into the cell cycle, maintain continuous proliferation, maintain the viral genome in a latent episomal form, and probably prevent cells from undergoing terminal differentiation or apoptosis.

gp-350: a late virion protein that is used as a marker of the lytic program; this antigen is expressed at the cell surface.

Infectious Mononucleosis (IM): Symptoms of IM include fever, sore throat, swollen glands, and sometimes swollen spleen or liver. Cardiovascular and Central Nervous Systems involvement rarely occurs, and IM is almost never fatal.

Lytic cycle: lysis of host cell by a virus releasing progeny that infect other host cells and repeat the cycle.

Mutu-I BL cells: clones of Burkitt's Lymphoma cell line.

Phorbol ester PMA: Phorbol 12-myristate 13-acetate; activates protein kinase C; functions to induce the lytic cycle.

Propidium Iodide (PI): fluorescent compound used to determine DNA content.

TGF-b: immunomodulatory cytokine transforming growth factor-beta; functions to induce the lytic cycle.

Western Blot Analysis: technique used to detect the presence of a specific protein in a mixture. The proteins are separated by gel electrophoresis and transferred to a nitrocellulose or nylon filter that is incubated with a specific antibody. Autoradiography is used to locate antibody/protein binding.