THE SECOND STEP: Making U87-CD4-CCR5 cells

Human glioma a tumor arising from neuroglia cells (U87) are transfected to express
human CD4 " cluster of differentiation 4" marker on the surface of the host cell which is the primary binding receptor for HIV and CCR5 (CC chemokine receptor 5) Upon binding to CD4, the HIV envelope protein changes so it can then interact with one of several coreceptors including CCR5 or CXCR4 before further changes take place in the viral envelope protein , which are the major receptor and one of the coreceptors for this HIV-1 isolate, respectively.

These cells are referred to as U87-CD4-CCR5 cells.

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