Neutralization by FC and FI vaccine sera in assays with human primary blood lymphocytes (PBLs)
Figure 2B
Human primary blood lymphocyes were immunized with FC immunogen (black squares;n=4)( Asterisks indicate p24 antigen concentrations below the limit of detection at the dilution used in the ELISA.) , FI immunogens(COS-env with U87 cells, gray circles,n=4; COS-env with U87-CD4 cells, gray diamonds,n=3; COS-env with sCD4, white diamonds,n=2; COS-env with U87-CD4-CCR5 cells, each fixed separately before mixing for immunization, gray squares,n=2), or mock-transfected COS cell immunogen (cocultured with U87-CD4-CCR5 cells)(white circles;n=2). Unimmunized control mice (white triangles;n=2) were also used.
Figure 2B Interpretation In marked contrast to FC immunogens, all FI immunogens were unable to elicit significant neutralization of the homologous PI virus
How would you compare the results using the transgenic mice (click here to figure 2A, then click back to return) and the human primary blood lymphocytes (PBLs) (figure 2B above) Answer The difference in neutralization by FC and FI vaccine was observed in both
NEXT QUESTION TO PONDER:
Could the FC vaccine sera inhibit infectivity of pseudotyped HIV virions bearing an amphotropic murine leukemia virus (MLV) envelope protein or a PI of the simian immunodeficiency virus SIVmac251?Click here to find out!
Click here to go back to Main Menu