Could FI vaccines elicit neutralization of a related laboratory-adapted isolate of HIV, the T cell line-adapted derivative of 168P, 168C?

Neutralization sensitivity of the 168P PI virus and its TCLA derivative 168C were tested in U87-CD4-CXCR4 cells with pooled sera: FC immunogen (black squares), FI immunogens (COS-env + U87 cells, gray circles; COS-env + U87-CD4 cells, gray diamonds; COS-env + sCD4, white diamonds), and mock-transfected cell controls (white circles).

 

Interpretation of these results:

 Were neutralization titers of the TCLA 168C virus comparable between FC and FI vaccine sera ? Answer Yes

Remember this was also true for the titers of antibodies to gp120 ( anti-gp120).

What does this suggest? Answer A similar degree of inherent immunogenicity among the vaccines.

Check for understanding.

If the FC vaccines elicited PI virus neutralization where the FI vaccines failed, what does this suggest?
Answer FC immunogens present unique fusion-dependent determinants that mediate neutralization of PI viruses.

What next? Click here to determine the breath of PI virus neutralization elicited by FC immunogens.

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