Virus	Titer (PFU/ml)
Wild type	1.9 × 105
M2V27T	6.0 × 105
M2A30P	1.1 × 106
M2S31N	1.2 × 106
M2del29-31	1.7 × 106
M2HATM	2.2 × 104
M2NATM	2.2 × 103
DM2TMCYT	1.4 × 104

Note: Yellow highlights reflect the point mutation mutants

TABLE 1. Virus titers in the supernatant of 293T cells after plasmid transfection  

**More than 10⁵ transfectant viruses with the wild-type or mutant M gene were present. All viruses that bear the M2 mutation as well as the wild type virus generated similar efficiencies.

What conclusions can you draw?

  Answer Definite conclusions cannot be drawn from this experiment The data so far supports the notion that without an intact M2 protein, Influenza A can still replicate in vitro