Table 2-Replication of M2 mutants in mice

Virus
Nasal turbinate
Lung
Wild-type

3.9 ± 0.5

6.8 ± 0.3
M2V27T

4.3 ± 0.7

7.3 ± 0.3
M2A30P

NRb

6.8 ± 0.1
M2S31N

4.3 ± 0.4

7.0 ± 0.2
M2del29-31

NR

5.6 ± 0.1
M2HATM

NR

NR
DM2TMCYTc

NR

NR

Results obtained are measured as follows: Mean titer (log10 PFU/g) ± SD

bNR, virus not recovered from any of the infected mice (less than 102 PFU/g)

c Mice were infected intranasally with 50 µl of virus (7 × 102 PFU).

 
**All mutants that showed ionic activity replicated in the mice.

**Replication in the lungs of the M2del29-31 mutant was lower by more than 10 fold from the other mutants

**Showed that M2 ion channel activity is necessary for efficient influenza a virus replication in vivo

 

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