Are reovirus ts mutants with defined blocks in viral replication capable of inducing apoptosis?

RESULTS

Every ts mutant demonstrated a defect in the production of viral progeny at the non-permissive temperature.

T3D and each of the ts mutants were capable of inducing apoptosis at the permissive and nonpermissive temperatures. However, tsC447 and tsG453 induced 50 % fewer cells to undergo apoptosis at the nonpermissive temperature.

The PARP assay confirmed that at the non-permissive temperature tsC447, tsD357, and tsG453 induce apoptosis with similar efficiency compared to the wild type (T3D).

QUESTIONS

Temperature sensitive mutants grew at the same rates at the permissive and nonpermissive temperatures.

TRUE Incorrect

FALSE Correct

EXPLANATION The overall results showed that viral yield is greater at the permissive temperature.

These experiments proved that reovirus replication after disassembly is required to induce apoptosis.

TRUE Incorrect

FALSE Correct

EXPLANATION The ts mutants have defined block in viral replication. However, these mutant are still capable of inducing apoptosis at the permissive and nonpermissive temperatures.

The PARP experiment was done to support the results obtained from the first experiment (figure A).

  TRUE Incorrect. The PARP assay was done to support the results obtained from figure B.

FALSE Correct. The PARP assay was done to support the results obtained from figure B.

EXPLANATION The PARP experiment was performed to eliminate the variable of time, since ts mutants may not be able to intiate a second round of replication at the nonpermissive temperature.

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