Does treatment of cells with inhibitors of reovirus disassembly block apoptosis induced by virions?

RESULTS

Inhibitors of reovirus disassembly inhibit viral growth?

TRUE Correct. Inhibitors of reovirus disassembly inhibit viral growth.

FALSE Incorrect. Inhibitors of reovirus disassembly inhibit viral growth.

EXPLANATION The growth of T3SA+ virions in HeLa cells incubated in the absence of either AC or E64, had higher viral yields than T3SA+ virions grown in cells incubated in the presence of these inhibitors.

Do inhibitors of reovirus disassembly block apoptosis?

YES Correct. Inhibitors of reovirus disassembly block apoptosis.

NO Incorrect. Inhibitors of reovirus disassembly block apoptosis.

EXPLANATION T3SA+ virions induced 47 % of HeLa cells to undergo apoptosis in the absence of either AC or E64; however, apoptosis was reduced to the level of mock-infected cells in the presense of these inhibitors.

TNF-a does not induce apoptosis in AC- or E64-treated cells.

TRUE Incorrect. TNF-a induces apoptosis in AC- and E64-treated cells.

FALSE Correct. TNF-a induces apoptosis in AC- and E64-treated cells.

EXPLANATION TNF- a, a known inducer of apoptosis, was used as a positive control. This control proves that AC and E64 do not block the activity of cellular proteins, which are required for apoptotic cell death.

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