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Introduction and basic lab techniques

Stains

Dilutions

Info for group projects

Module 1

Module 2

Other resources

safety procedures

The lab notebook

Bacterial isolation from the environment

Aseptic technique

Using the bright field microscope

Pure culture techniques: streak plate and spread plate

Stains

Smear preparation/simple stain

Negative stain (Capsule stain)

Preparation of stock and working cultures

Gram stain

Dilutions

 

Info for group projects:

Group Project

Reading the Primary Literature

Preservatives effectiveness - Designing an Experiment

 

MODULE 1: How is the microbial community within a sample determined?

Media

Anaerobic culture techniques

Live-dead stain

Fluorescence microscopy

Direct count quantitation

Quantification methods

Motility

Identification tests:

-Fermentation reactions

-Enzyme production

-Single-media/multiple tests

-Multiple and rapid test systems

-Ribosomal RNA analysis

How to design a Table; graphing data

Unknowns

Genetic analysis of unknowns

Polymerase Chain Reaction – simulation: http://www.hhmi.org/biointeractive/vlabs/index.htm

MODULE 2: What factors affect microbial growth and gene expression?

Ames test

Biofilm Formation

Bacteriophage quantitation

 

OTHER INTERNET RESOURCES

American Type Culture Collection (ATCC)

American Society for Microbiology

Microbiology Courses at

Catonsville Community College

University of Texas

Washington State University

RESOURCES IN THE LAB

• DIFCO Manual
• HUGO - PC manual of media by Hardy Diagnostics
• Bergey's Manual of Determinative Bacteriology
• Bergey's Manual of Systematic Bacteriology

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